Looking for a research position

Summary:

I'm attaching a CV for Dr. Jing Huang. She is an excellent scientist, but her current funding is ending and she is looking for another position. If anyone needs a hard working individual, I would recommend her. Tony Bakke

Looking for a research position

Tony12 05 May 2010 21:30

JING HUANG, PHD.
10765 SW Washington • Portland, OR 97225
ude.usho|ijgnauh#ude.usho|ijgnauh or moc.liamg|8ijgnauh#moc.liamg|8ijgnauh • 503.998.6858 (c)
Curriculum Vitae
EDUCATIONAL BACKGROUND
OREGON STATE UNIVERSITY, Corvallis, OR
Doctor of Philosophy (PhD) in Genetics, 1998 –Minor in Biochemistry

PORTLAND STATE UNIVERSITY, Portland, OR
M.B.A. in Finance and Marketing, Dec. 2006
Applied Statistics Certificate, 2006 (unfinished)

EXPERIENCE HIGHLIGHTS
• Cancer Vaccine and immunology (current) July, 2008-current
--Dr. Craig Okada lab, OHSU
o Target disease-T cell lymphoma
o Strategy- create and generate anti-human T cell receptor antibody by engineering mouse human chimeric T cell receptor
o Major techniques—
 Create mouse-human chimeric TCR (T cell receptor)
 Test TCR COS cells expression by ELIZA
 Generate stable high expression TCR producer in BW cells
 Purify ultra-pure TCR protein by
 Generate monoclonal anti-human TCR antibody
 Test antibody-antigen interation by flow cytometry
o Achievement and /or progresses -publication in process
A role of isotype 2c in C6VL TCRid-targeted tumor immunotherapy

• Chromosome Translocation Study (Dec. 2003-July 2008)
--Dr. Elliot Epner lab, The Knight Cancer Institute, OHSU
o Target disease-B cell lymphoma
o Strategy— identify major factors and establish a molecular model (base) for chromosome translocation related B cell lymphoma
o Major techniques
 Identify chromosome translocation related diseases Chromsome immunoprecipitation (CHIP)
 Identify molecular base for CHIP findings by Sorthern and bisulfite assay
 Identify celllular location on target genes and interactions by FISH and immunocytochemistry
 Knockout target gene expression by siRNA
 Detect functionalities on knockout genes by appoptosis assay
 Establish target gene expression pattern by RT-PCR
 Immunoblot target gene proteins
o Achievements and /or Progresses—- major results published in
 Liu, H. Huang, J., et al, “Transvection Mediated by the Translocated Cyclin D1 Locus in Mantle Cell Lymphoma,” Journal of Experimental Medicine, 2008 July 14. ( First two authors are equally contributed to the paper)
 Huang, J. et al, “Cyclin D3 can compensate for Cyclin D1 in t(11;14) B cell malignancies,” submitting.

• Identify Biomarkers ( Nov 2002 to Nov 2003)
-— ProteoGenix. INC
o Target diseases- Downs syndrome and amniotic infections
o Strategy-identify early-stage-pragnance blood and amniotic flow biomarkers for Downs Syndrome and amniotic infections
o Major techques——
 Identify biomarkers by running two dimensional protein gel
 Identify target protein sequence by LC mass spectrometer
 Identify target protein expression pattern by Surface-Enhanced Laser Desorption/Ionization (SELDI)
 Generate antibodies for potential biomarker candidates
 Screen high-quality antibodies by western blots
 Develop assay kits for potential candidates
o Achievements and /or Progresses—the products went on for clinical trial

• DNA repair and cell cycle checkpoints in cancer (Aug. 1998 to Oct. 2002)
• -Dr. Matt Thayer lab, Oregon Health & Science Institute
o Target diseases- General oncology
o Strategyestablish a molecular model for DNA demage and repair checkpoints
o Major techniques
 Identify key players in DNA demage checkpoints by  irradiation
 Immunoblot target proteins following irradiation
 Study target-proteins changes and interactions by transient transfections, followed by illucifience assy.
 In vitro and in vivo kinase assay
 Engineering gene and proteins
 Protein purifications
 FISH and immunocytochemistry
 Cell cycle in vivo labling
o Achievements and /or Progresses—major results published in
 Stauffer, D., Change, B., Huang, J., Dunn A., Thayer M., “CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint,” J Bio Chem 282, number 13 (2007)
 Change B.H., Smith, L., Huang, J., Thayer, M., “Chromosomes with delayed replication timing lead to checkpoint activation, delayed recruitment of Aurora B and chromosome instability,” Oncogene 22;26 number 6 (2007),1852-61

RESEARCH AREA SUMMARY:
Study of T cell Lymphoma (current)
• produce cancer vaccine for T cell Lymphoma
Study of Mantle Cell Lymphoma and other related blood cancers (Dec.2003 to Present)
 Investigated cyclin D1 and D3 involvement in Mantle Cell Lymphoma development and screened potential drugs for ability to damage cancer cells and potentially cure cancer.
Study to identify biomarkers in Down Syndrome and intra-amniotic infection (Nov. 2002 to Nov. 2003)
 Performed analysis of pre-natal amniotic fluid and blood to determine amniotic and serum biomarkers, allowing for early detection of fetal downs syndrome and maternal amniotic infection.
Study of ATR role and interaction with p300 in cell cycle regulation and cancer development ( Aug. 1998 to Oct. 2002)
 Investigated the regulation of p53 function by ATR and mdm2 in mouse and mammalian cells to determine ATR involvement (both alone and in conjunction with p300) in cellular DNA replication and damages.
 Explored the role of ATR/p300 in cancer formation and development.

Study of muscle protein degradation (July 1993 to Apr.1998)
 Studied mechanism of muscle protein degradation and muscular diseases and determined how this process is mediated by the Calpain family
Study of effect of irradiation on plant DNA (September 1992 to September 1993)
 Participated in identification of plant DNA damage and repair pathways following U.V. irradiation to identify DNA damage factors for humans.

PUBLICATIONS
1. Huang, J. et al, “Cyclin D3 can compensate for Cyclin D1 in t(11;14) B cell malignancies,” submitting
2. Huang, J., Liu, H., et al, “Transvection Mediated by the Translocated Cyclin D1 Locus in Mantle Cell Lymphoma,” Journal of Experimental Medicine, 2008 July 14.
3. Stauffer, D., Change, B., Huang, J., Dunn A., Thayer M., “CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint,” J Bio Chem 282, number 13 (2007)
4. Change B.H., Smith, L., Huang, J., Thayer, M., “Chromosomes with delayed replication timing lead to checkpoint activation, delayed recruitment of Aurora B and chromosome instability,” Oncogene 22;26 number 6 (2007),1852-61
5. Huang, J. & Forsberg, N.E., ”Role of calpain in skeletal muscle protein degradation,” Proc. Natl. Acad. Sci. USA 95 (1998), 12100-12105
6. Ueda, Y., Wang, Mei-chan, Ou, B.R., Huang, J., Elce, J., Tanaka, K.., Ichihara, A., and Forsberg, N.E., “Evidence for the participation of the proteasome and calpain in early phases of muscle cell differentiation,” Biochem. Cell Biol. 30 (1998), 679-694
7. Huang, J., & Forsberg, N.E. “Genetic analysis of calpain isoform function in skeletal muscle: new strategies to study protease function.” In Calpain: Pharmacology and Toxicology of a Calcium-Dependent Cellular Protease. Ed. Kevin K., W. Wang, and Po-wai Yuen: Taylor and Francis
8. Forsberg, N.E., & Huang, J. “Strategies to regulate calpain activities in living cells and transgenic animals,” in Calpain Methods and Protocols: Methods in Molecular Biology. Ed. John Elce: Humana Press
9. Huang, J. & Thayer, M. “Interaction between cell cycle factors and muscle differentiation program. Stem Cells and Cell Signaling in Skeletal Myogenesis,” in Advances in Developmental Biology and Biochemistry, Volume 6. Ed. David Sassoon